Silica coating of luminescent quantum dots prepared in aqueous media for cellular labeling

• We present a facile modified Stöber method to prepare highly luminescent QD@SiO2.
• The PL efficiency of QDs increases significantly after silica coating.
• QD@SiO2 exhibits small size (∼5 nm) and great dispersibility in aqueous solution.
• QD@SiO2 presents extraordinary photo and colloidal stability.
• The silica shell eliminates QD cytotoxicity, providing the access of bioconjugation.

Silica coating is an effective approach for rendering luminescent quantum dots (QDs) with water dispersibility and biocompatibility. However, it is still challenging to prepare silica-coated QDs (QD@SiO2) with high emission efficiency, small size and great stability in favor for bioapplication. Herein, we reported a modified Stöber method for silica coating of aqueously-prepared CdTe/CdS QDs. With the coexistence of Cd2+ and thioglycolic acid (TGA), a thin silica shell was formed around QDs by the hydrolysis of tetraethyl orthosilicate (TEOS). The resultant QD@SiO2 with a small size (∼5 nm in diameter) exhibits significantly higher emission efficiencies than that of the initial QDs. Also, QD@SiO2 has extraordinary photo and colloidal stability (pH range of 5–13, 4.0 M NaCl solution). Protected by the silica shell, the cytotoxicity of QDs could be reduced. Moreover, the QD@SiO2 conjugated with folic acid (FA) presents high specific binding toward receptor-positive HeLa cells over receptor-negative A549 cells.

A facile route based on modified Stöber method was used for the synthesis of silica coated QDs (QD@SiO2) starting from aqueously prepared CdTe/CdS QDs. The resultant QD@SiO2 exhibited a significant increase in emission efficiency compared with that of the initial QDs, along with a small size (∼5 nm in diameter), great stability and low cytotoxicity, which makes it a good candidate as robust biomarker.Figure optionsDownload as PowerPoint slide