کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1488835 | 992296 | 2013 | 8 صفحه PDF | دانلود رایگان |
• Molecular approach of synthesis of Fe2O3-NC using goat blood as a bio-precursor.
• The method is simple, efficient and environment friendly.
• Synthesized nanocrystals were characterized by UV–vis spectroscopy, XRD, SEM, TEM, DLS and EDS.
• Nanocrystals exhibited potent cytotoxicity against A549 cancer cell.
• Nuclear targeting with expression of caspase-3, caspase-7 and Bcl-2 in A549 cancer cells.
In this study, we synthesised iron oxide nanocrystals (Fe2O3-NC) from goat blood (bio-precursor) using red blood cells (RBC) lysis method (a molecular level approach) for the first time. The formation of Fe2O3-NC was achieved through a single-phase chemical reduction method. The size distribution of Fe2O3-NC falls between 20–30 nm for pellet and 100–200 nm for lysate and were found to be crystalline. Fe2O3-NC demonstrated significant cytotoxicity on A549. We report the direct visualization of interactions between Fe2O3-NC and the cancer cell nucleus. The active transport of Fe2O3-NC to the nucleus induces major changes to nuclear phenotype via nuclear envelope invaginations. We further examined the root cause for the involvement of Fe2O3-NC on the expression of caspase-3, caspase-7 and Bcl-2 in A549 cancer cells. This functional proteomic analysis clearly implies that the lung cancer cell proliferation is perfectly targeted by the biosynthesised Fe2O3-NC which could provide new insight for nuclear-targeted cancer therapy.
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Journal: Materials Research Bulletin - Volume 48, Issue 9, September 2013, Pages 3164–3171