Synthesis and electrochemical characterization of myoglobin-antibody protein immobilized self-assembled gold nanoparticles on ITO-glass plate

► The Mb-Ab was covalently bonded to carboxyl groups of the mixed MUA and MPA over self-assembled GNPs. ► This assembly on ITO-glass plate was used as an impedimetric immunosensor for myoglobin detection. ► High loading of Mb-Ab, on the GNPs results in a wide range of Mb-Ag detection from 0.01 μg to 1.65 μg mL−1 ► The simple method and wide range of Mb-Ag detection makes this advantageous over other methods.