Photorefractive digital holographic microscopy applied in microstructures analysis

In this work, we present a Photorefractive Digital Holographic Microscopy (PRDHM) technique based on the writing–reading holographic process in photorefractive Bi12TiO20 (BTO) crystal and the obtainment of phase and amplitude of the object wave were performed by a digital holographic method. We demonstrate that the wave diffracted by a photorefractive hologram recorded in a BTO crystal can be combined with a reference wave to record a second hologram in a CCD sensor in a configuration of digital holographic microscopy. The experimental measurements were performed on samples like red blood cells and a thin film structure, and were obtained quantitative values of amplitude and phase of the object wave, as well as 3D graphs, of the analyzed samples by the digital reconstruction holographic method. This technique presents a new method in replacement of the usual methods for reconstruction of holograms recorded in a photorefractive crystal and also presents the possibilities to obtain 3D phase images for surfaces characterization and applications in dynamic holography.