کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1539350 996634 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of molecular orientation of fluorescently-labeled duplex DNA on emission anisotropy
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد مواد الکترونیکی، نوری و مغناطیسی
پیش نمایش صفحه اول مقاله
Effects of molecular orientation of fluorescently-labeled duplex DNA on emission anisotropy
چکیده انگلیسی
The molecular orientation of Cy5-labeled target DNA hybridized on a probe DNA layer was studied on a planar waveguide in the Kretchmann configuration at various target DNA bulk concentration. Optical waveguide fluorescence spectroscopy (OWFS) was employed to evaluate the fluorescence emission anisotropy by analyzing two polarized modes, i.e., TM and TE modes propagating in a waveguide. In this way, the orientational order parameter S could be determined. It was found that the orientation of DNA duplex molecules is disordered at lower concentrations, while relatively ordered at higher concentrations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Optics Communications - Volume 282, Issue 5, 1 March 2009, Pages 1062-1066
نویسندگان
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