کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1540826 | 996668 | 2009 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: In vivo imaging of dynamic biological specimen by real-time single-shot full-field optical coherence tomography In vivo imaging of dynamic biological specimen by real-time single-shot full-field optical coherence tomography](/preview/png/1540826.png)
We demonstrate the feasibility of a compact single-shot full-field time domain optical coherence tomography (OCT) for imaging dynamic biological sample in real-time. The system is based on a Linnik type polarization Michelson interferometer and a four-quadrature phase-stepper optics, which can simultaneously capture four quadraturely phase-stepped interferograms on a single CCD. Using a superluminescent diode as light source with center wavelength of 842 nm and spectral width of 16.2 nm, the system yields an axial resolution of 19.8 μm, and covers a field of view of 280 × 320 μm2 (220 × 250 pixels) with a transverse resolution of 4.4 μm by using a 10× microscope objective (0.3 NA). Three-dimensional OCT images of biological samples such as an onion slice and a diaptomus were obtained without any image averaging or pixel binning. In addition, in vivo depth resolved dynamic imaging was demonstrated to show the beating internal structure of a diaptomus with a fame rate of 5 fps.
Journal: Optics Communications - Volume 282, Issue 4, 15 February 2009, Pages 674–683