کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1541117 996674 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Field confinement with aberration correction for solid immersion lens based fluorescence correlation spectroscopy
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد مواد الکترونیکی، نوری و مغناطیسی
پیش نمایش صفحه اول مقاله
Field confinement with aberration correction for solid immersion lens based fluorescence correlation spectroscopy
چکیده انگلیسی
The solid immersion lens (SIL) as a tool for increasing the field confinement as well as providing optimal performance by aberration compensation in a confocal fluorescence correlation spectroscopy (FCS) system is illustrated here. Using Zernike polynomials we show that aberration compensation and the resultant pre-shaping of the incident wavefront enables near diffraction-limited performance. This is explained based on vectorial computations for high apertures in the Debye approximation. The obtained axial resolution parameters are compared with the obtained diffusion times in a SIL-FCS experiment for measurements in solutions done at the single molecule level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Optics Communications - Volume 271, Issue 2, 15 March 2007, Pages 462-469
نویسندگان
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