کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1541357 | 996680 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Arrangement of a 4Pi microscope for reducing the confocal detection volume with two-photon excitation Arrangement of a 4Pi microscope for reducing the confocal detection volume with two-photon excitation](/preview/png/1541357.png)
The main advantage of two-photon fluorescence confocal microscopy is the low absorption obtained with live tissues at the wavelengths of operation. However, the resolution of two-photon fluorescence confocal microscopes is lower than in the case of one-photon excitation. The 4Pi microscope type C working in two-photon regime, in which the excitation beams are coherently superimposed and, simultaneously, the emitted beams are also coherently added, has shown to be a good solution for increasing the resolution along the optical axis and for reducing the amplitude of the side lobes of the point spread function. However, the resolution in the transverse plane is poorer than in the case of one-photon excitation due to the larger wavelength involved in the two-photon fluorescence process. In this paper we show that a particular arrangement of the 4Pi microscope, referenced as 4Pi′ microscope, is a solution for obtaining a lateral resolution in the two-photon regime similar or even better to that obtained with 4Pi microscopes working in the one-photon excitation regime.
Journal: Optics Communications - Volume 264, Issue 1, 1 August 2006, Pages 123–129