کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1548643 997749 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
MicroRNA (miR396) negatively regulates expression of ceramidase-like genes in Arabidopsis
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد مواد الکترونیکی، نوری و مغناطیسی
پیش نمایش صفحه اول مقاله
MicroRNA (miR396) negatively regulates expression of ceramidase-like genes in Arabidopsis
چکیده انگلیسی

MicroRNAs (miRNAs) are 21–23 nucleotide (nt), endogenous RNAs that regulate gene expression by targeting mRNAs for direct cleavage or translational repression in plants. In Arabidopsis, miR396 is encoded by two different loci (MIR396a and MIR396b) and sequence analysis suggests it may target three ceramidase-like genes (Atceramidase-like 1, Atceramidase-like 2 and Atceramidase-like 3). To demonstrate the biological function of miR396, we inserted the synthetic precursors, MIR396a or MIR396b, under the control of the enhanced cauliflower mosaic virus (CaMV) 35S promoter, into a plant transformation vector (pOCA30) and transformed the constructs into Arabidopsis. The promoter increased miR396 levels by more than 2-fold, indicating appropriate maturation of the synthetic precursor MIR396a or MIR396b transcript in transgenic plants. Microarray analysis showed that the transcript levels of two ceramidase-like genes (Atceramidase-like 1, Atceramidase-like 2) were decreased by more than 2-fold and lactosylceramide 4-α-galactosyltransferase increased by more than 2-fold in transgenic plants compared with the empty vector-transformed plants. Northern blot analysis showed that the mRNA levels of the two ceramidase-like genes were significantly reduced in transgenic plants. These results indicated that miR396 probably plays a crucial role in the ceramide metabolism pathway by negatively regulating the expression of ceramidase-like genes in Arabidopsis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Progress in Natural Science - Volume 19, Issue 6, 10 June 2009, Pages 781–785
نویسندگان
, ,