کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1548693 | 997752 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sodium current of neurosecretory cells in the eyestalk from the shrimp Penaeus japonicus
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
مواد الکترونیکی، نوری و مغناطیسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The properties of the inward current of medulla terminalis-X-organ (MTXO) cells isolated from the Penaeus japonicus eyestalk were studied with the whole-cell clamp technique in the presence of Ca2+ and K+ channel blockers. The inward currents had a threshold at about â50 mV and peaked at â10 mV. The reversed potential (Vrev) was very close to VNa, the theoretical Nernst equilibrium potential for Na+. Vrev followed VNa when the external Na+ concentration was varied and the currents were entirely suppressed by 30 nM tetrodotoxin (TTX), indicating that it was carried by Na+. The smooth line of concentration-dependent inhibition of sodium currents by TTX represented the best fit with the Hill equation, yielding an IC50 of 2.1 ± 0.1 nM. The values of the half-maximal activation voltage Vh were â20.6 ± 0.5 and â19.3 ± 0.5 mV, respectively, in the absence and presence of 2 nM TTX. TTX had no significant effect on the voltage dependence of steady-state activation and inactivation of INa. Taken together, the results suggest that the inward current recorded under our experimental conditions was carried by sodium ions flowing through fast voltage-dependent Na+ channels.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Progress in Natural Science - Volume 19, Issue 11, 10 November 2009, Pages 1493-1499
Journal: Progress in Natural Science - Volume 19, Issue 11, 10 November 2009, Pages 1493-1499
نویسندگان
Jinshen Liu, Shiying Li, Haihui Ye, Guizhong Wang,