کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1549075 | 997771 | 2008 | 5 صفحه PDF | دانلود رایگان |
Microarray analysis is used to identify transcriptional targets. However, the direct targets of transcription factors cannot be distinguished from indirect ones; further, genes with low-expression levels cannot be identified by this method. In the present study, we exploit the cis-element dehydration-responsive element (DRE) that is known to be responsible for the transcription of DRE binding factor 1 (DREB1) targets in the promoter region of all Arabidopsis genes. Putative targets whose promoters contain the elements were verified by both microarray and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Five new DREB1/CBF direct targets were identified. Compared with traditional microarray analysis, our method is convenient and cost-effective for identifying the downstream targets of transcription factors.
Journal: Progress in Natural Science - Volume 18, Issue 5, 10 May 2008, Pages 579–583