Identifying bacterial fragments on morphologically similar substrate using UAFM

The ultrasonic atomic force microscopy (UAFM) can be used effectively to map the elasticity of a surface. Using this technique we have demonstrated that biological fragments on a substrate can be easily identified which is otherwise difficult using only an AFM image. We have shown that AFM image can falsely interpret the surface morphological features on the substrate. We have taken the bacteria Pseudomonas sp. as a case study to demonstrate that UAFM technique is a powerful tool to study biological samples and differentiate morphological features on the substrate.