کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1589281 1001984 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct visualization of the formation and structure of RecA/dsDNA complexes
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد دانش مواد (عمومی)
پیش نمایش صفحه اول مقاله
Direct visualization of the formation and structure of RecA/dsDNA complexes
چکیده انگلیسی
A direct visualization of the construction of RecA/DNA nucleofilament complexes was obtained using high-resolution atomic force microscopy (AFM). It showed that the complex consisted of helical loops, with each helical loop composed of units of size equivalent to ∼six monomers. The contour of stretched DNA that was embedded beneath RecA monomers was also observed directly. Images of RecA/DNA complexes showed both left- and right-handed helices, with a range of helical pitches. This was considered to imply that the complexes were flexible enough such that they are prone to compression forces that occur during sample preparation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Micron - Volume 41, Issue 3, April 2010, Pages 227-231
نویسندگان
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