کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1589520 | 1001997 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structured illumination microscopy of autofluorescent aggregations in human tissue
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
دانش مواد (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Structured illumination microscopy of autofluorescent aggregations in human tissue Structured illumination microscopy of autofluorescent aggregations in human tissue](/preview/png/1589520.png)
چکیده انگلیسی
Sections from human eye tissue were analyzed with Structured Illumination Microscopy (SIM) using a specially designed microscope setup. In this microscope the structured illumination was generated with a Twyman-Green Interferometer. This SIM technique allowed us to acquire light-optical images of autofluorophore distributions in the tissue with previously unmatched optical resolution. In this work the unique setup of the microscope made possible the application of SIM with three different excitation wavelengths (488, 568 and 647 nm), thus enabling us to gather spectral information about the autofluorescence signal.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Micron - Volume 42, Issue 4, June 2011, Pages 330–335
Journal: Micron - Volume 42, Issue 4, June 2011, Pages 330–335
نویسندگان
Gerrit Best, Roman Amberger, David Baddeley, Thomas Ach, Stefan Dithmar, Rainer Heintzmann, Christoph Cremer,