Evaluation of Bactericidal Activity of Biologically Synthesised Silver Nanoparticles from Candida albicans in Combination with Ciprofloxacin

The aim and objective of present study was biosynthesis silver nanoparticles from fungi Candida albicans. The fungal biomass was challenged with 1 mM AgNO3, which results in the colour change of the solution indicates the formation of Silver nanoparticles. These nanoparticles were further characterized by UV- visible spectroscopy which showed the absorption peak at 430 nm. The FT-IR spectrum revealed the binding of protein to nanoparticles, which may be stabilized by the silver nanoparticles. The FESEM and AFM analysis showed nanoparticles are spherical and their size ranges in 60.88-65.57 nm. The nanoparticles were further evaluated for its bactericidal activity against selected pathogenic bacteria singly and in combined form with Ciprofloxacin. The relative importance of particle-specific effect silver nanoparticles, in combination with Ciprofloxacin has shown enhanced bacterial activity. Thus these silver nanoparticles may prove as better candidates for drugs and can potentially eliminate the higher doses of antibiotics.