A facile method to prepare polysaccharide-based in-situ formable hydrogels with antibacterial ability

• A novel and facile one-pot approach is proposed to fabricate polysaccharide-based in-situ formable hydrogels.
• The method proposed can provide important implications for the design of other bioinspired materials.
• Physical and chemical properties of the hydrogels can be tuned by the molar ratio of free thiol/vinyl groups.
• The hydrogels presented antibacterial activities against Staphylococcus aureus and Escherichia coli.

Polysaccharide-based in-situ formable composite hydrogels were prepared by a facile one-pot approach via Michael addition reaction, with maleilated chitosan and thiol derivatised hyaluronan. The hydrogels were characterized by Fourier transform infrared spectroscopy, Scanning electron microscopy, swelling ratio, oscillatory rheology and antibacterial activity. Results showed that the hydrogels with different molar ratios of free thiol/vinyl all formed in-situ within 15 min. With increasing of vinyl contents, gelation time and storage modulus increased, while porosity and swelling ratio decreased. Additionally, the hydrogels presented antibacterial activities against Staphylococcus aureus and Escherichia coli, which will be benefit for biomedical applications.

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