Anti-cMet antibody conjugated hollow gold nanospheres as a new nano-material for enhancing the effect of photothermal therapy

• We synthesize HGNs with an NIR absorption peak of specific wavelength.
• Pegylation is used to combine HGNs with the monoclonal antibody of cMet.
• We prove cMet overexpresses on the membrane of HeLa and CaSki by IF.
• NIR with presence of anti-cMet conjugated HGNs induces a growth in apoptosis rate.
• Laser irradiation leads cancer cells apoptosis by disrupting Bcl-2/Bax balance.

The aim of our study was to investigate whether anti-cMet antibody conjugated hollow gold nanospheres (anti-cMet/HGNs) could enhance the sensitivity of photothermal ablation therapy on cervical cancer. Photothermal therapy in vitro was conducted on HeLa and CaSki using near-infrared ray (1.7 J/cm2) in four groups: control, laser only, hollow gold nanospheres (HGNs) with laser and anti-cMet/HGNs with laser. Compared with other groups, group of anti-cMet/HGNs with laser possessed a higher early apoptotic rate by 5.83% in HeLa and 20.0% in CaSki. Meanwhile, this group indicated an overexpression of Caspase-3, Bax and Cyclin E2. Anti-cMet/HGNs had potentials to enhancing the effect of photothermal therapy on cervical cancer by disrupting the balance between Bcl-2 and Bax.

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