Biosynthesis of silver nanoparticles using Tithonia diversifolia leaf extract and their antimicrobial activity

• Synthesis of silver nanoparticles (AgNPs) using Tithonia diversifolia leaf extract.
• Characterization of AgNPs using UV, TEM, EDX, TG-DTA, and FT-IR spectrum.
• Antimicrobial activity of AgNPs against P. aeruginosa, M. foliorum, B. subtilis, and R. equi.

A green approach for the synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of Tithonia diversifolia under ambient conditions was studied. The effect of pH on biosynthesis of AgNPs was investigated by UV–vis spectrophotometer. TEM images showed that the diameter of stable AgNPs was approximately 25 nm. TG-DTA analysis revealed that content of biomolecules surrounding silver was about 24.2%. Moreover the antimicrobial activity of synthesized AgNPs against Pseudomonas aeruginosa, Microbacterium foliorum, Bacillus subtilis, and Rhodococcus equi was screened.