New semi-IPN scaffolds based on HEMA and collagen modified with itaconic anhydride

Porous semi-IPN scaffolds were synthesized via free radical copolymerization of collagen modified with itaconic anhydride and 2-hydroxyethyl methacrylate, using ammonium persulfate and N,N,N′,N′-tetramethylethylenediamine as redox initiator system, with the aim of overcoming the high enzymatic degradation rate of native collagen scaffolds. The chemical modification of collagen was confirmed by 1H NMR and FT-IR spectroscopy. The physico-chemical properties of the resulted matrices were investigated by elemental analysis, SEM, enzymatic degradation and water retention studies. The synthesized scaffolds, obtained through an innovative process, have fine microstructures, controlled water retention degree and enhanced stability against enzymatic digestion compared to native collagen. This may broaden the use of collagen-based scaffolds in tissue engineering, particularly for wound dressings.

► New semi-IPN scaffolds based on itaconic anhydride modified collagen and HEMA.
► The synthesis of the scaffolds does not involve chemical crosslinking agents.
► The obtained scaffolds have an open and interconnecting porosity.
► Water uptake and degradation are dependent by the final composition of the semi-IPNs.