Comparative adsorptive removal of biperidene and sibutramine chlorhydrates from methanolic solutions by using active coal, clay and polymeric resins

Different absorbent materials were evaluated for extraction of organic compounds from methanolic solution used for high performance liquid chromatography (HPLC). The active coal, Betonite K-10 clay, commercial resins, namely Amberlyst A26OH™ and Amberlyst 15WET™ and three synthesized resins: RP03 (based on acrylonitrile and divinylbenzene), RP09 (based on methacrylamide, styrene and divinylbenzene) and RP12 (based on methacrylic acid, styrene and divinylbenzene) were evaluated. These materials have been tested in the removal of sibutramine and biperidene chlorhydrates from methanol for different extraction times. The extractions had been carried out in batch at room temperature. The initial and final concentrations of sibutramine and biperidene chlorhydrates in methanol were determined by HPLC. In a general way, the synthesized and commercial copolymers have presented the very good extraction performance concerning chlorhydrates. Notably, the Amberlyst 15WET resin has presented the most efficient retention of both chlorhydrates compared to the other absorbents.