کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1652384 | 1517340 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biochemically active sol-gel glasses: The trapping of enzymes
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
فناوری نانو (نانو تکنولوژی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We describe the preparation and properties of a biochemically active sol-gel glass, obtained by the enzyme alkaline phosphate (ALP) in a polymerizing tetramethoxysilane. The immobilized purified ALP from bovine intestinal mucosa had a 30% activity yield and an improved stability to thermal deactivation compared to a solution. The composite bioactive glass was preserved in water at room temperature for two months without loosing activity. A non–Michaelis–Menten kinetics was observed. The concept of preparing bioactive materials by the sol-gel method seems to be general. Thus, other enzymes (chitinase, asparatase, β-glucose) were successfully trapped.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Letters - Volume 61, Issues 14–15, June 2007, Pages 2843–2846
Journal: Materials Letters - Volume 61, Issues 14–15, June 2007, Pages 2843–2846
نویسندگان
Sergei Braun, Sara Rappoport, Rivka Zusman, David Avnir, Michael Ottolenghi,