کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1678203 1009934 2007 18 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structure determination of tubular crystals of membrane proteins. IV. Distortion correction and its combined application with real-space averaging and solvent flattening
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد فناوری نانو (نانو تکنولوژی)
پیش نمایش صفحه اول مقاله
Structure determination of tubular crystals of membrane proteins. IV. Distortion correction and its combined application with real-space averaging and solvent flattening
چکیده انگلیسی

A method for correction of three-dimensional distortions has been developed for helical assemblies and applied to tubular crystals of Ca2+Ca2+-ATPase. This method approximates distorted helical particles with short straight segments of different orientation parameters, which are determined by fitting them to the reference data in reciprocal space. Thus, the method follows Beroukhim and Unwin [Ultramicroscopy 70 (1997) 57], but is more extended to achieve better alignment and to cope with images of poor S/N ratio. Substantial improvements were achieved by dividing the reference image into the segments of optimal lengths in exactly the same way as the test, and treating the distortions in the near and far sides of a helical particle separately. The improvement was further enhanced when combined with real-space averaging [Yonekura, Toyoshima, Ultramicroscopy 84 (2000) 15] and solvent flattening [Yonekura, Toyoshima, Ultramicroscopy 84 (2000) 29], and most pronounced when all these three were applied iteratively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultramicroscopy - Volume 107, Issue 12, November 2007, Pages 1141–1158
نویسندگان
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