کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1678941 1518370 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescence imaging and investigations of directly labelled chromosomes using scanning near-field optical microscopy
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد فناوری نانو (نانو تکنولوژی)
پیش نمایش صفحه اول مقاله
Fluorescence imaging and investigations of directly labelled chromosomes using scanning near-field optical microscopy
چکیده انگلیسی

 Scanning near-field optical microscopy (SNOM) has been successfully employed to generate high resolution (<100 nm) fluorescence images of directly tagged human chromosomes. Direct tagging, fluorescence in-situ hybridisation processes (with and without amplification) are investigated and their fluorescence response to near-field excitation are compared. Using the simultaneous topography mode of SNOM, chromosome morphology was seen to differ as a result of the two processes; with chromatin collapse more extensive when the amplified direct tagging procedure was used. The results are discussed in the context of developing locus specific direct tags together with high resolution SNOM imaging for the observation of chromosome aberrations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultramicroscopy - Volume 107, Issues 4–5, April–May 2007, Pages 308–312
نویسندگان
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