کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1687754 1010682 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nickel toxicity in the hepatopancreas of an isopod Porcellio scaber (Oniscidea)
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد سطوح، پوشش‌ها و فیلم‌ها
پیش نمایش صفحه اول مقاله
Nickel toxicity in the hepatopancreas of an isopod Porcellio scaber (Oniscidea)
چکیده انگلیسی

This study is focused on recognizing how the functional role of hepatopancreas, the main metal storage organ in woodlice, is affected by the excess of nickel, a toxic element to soil invertebrates. Chronic Ni toxicity (24 weeks) was studied on four groups of woodlice kept on dry shredded maple leaves contaminated with Ni at average concentrations of 0.1 μg g−1 (control), 8.0 μg g−1 (Ni1), 75 μg g−1 (Ni2) and 270 μg g−1(Ni3) dry weight. Micro-PIXE mapping of elemental distribution in the hepatopancreas of the Porcellio scaber woodlice was used to study relations between nickel and other elements in individuals exposed to different metal concentrations in the diet. Data were processed using GeoPIXE II software. Transmission electron microscopy (TEM) was used to check the relations between ultrastructural changes in hepatopancreatic cells and nickel tissue burden.Elemental mapping showed a dose-related nickel bioaccumulation in the hepatopancreas at concentrations from 3 μg g−1 (uncontaminated control animals) to nearly 840 μg g−1 (Ni3). Generally, nickel was distributed uniformly in small aggregations. A combined evaluation of elemental maps and electronograms showed that aggregations of nickel in arbitrarily selected micro-areas in PIXE maps could be the granular structures observed in TEM electronograms. The mechanism of Ni sequestration in the hepatopancreas could be similar to this used for cadmium or lead. The sequences of ultrastructural changes, which follow the increased burdens of Ni in the hepatopancreatic cells, were: the increase of intracellular electron-dense granules, increase in the number of myelin-like structures, intensified mitochondrial swelling and appearance of concentrically arranged, rough endoplasmic reticulum.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms - Volume 260, Issue 1, July 2007, Pages 213–217
نویسندگان
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