Improvement of adhesion of fucoidan on polyethylene terephthalate surface using gas plasma treatments

Attachment of polysaccharide fucoidan to the polyethylene terephthalate (PET) polymer surface was studied by X-ray photoelectron spectroscopy (XPS). Fucoidan has antithrombogenic and anticoagulant properties and is therefore a promising coating for vascular graft implants for improving their hemocompatibility. Samples of PET polymer were first modified by nitrogen plasma treatment in order to change the surface wettability and to introduce amino groups to the surface, which act as a linker for further binding of fucoidan. Plasma treated samples were then incubated for 30 min in fucoidan solution. The presence of fucoidan layer on the polymer surface was demonstrated by appearance of S2p signal in the XPS spectra of the coated PET samples. The procedure for immobilization of fucoidan on PET surface was optimized by varying pH value of fucoidan solution from 5 to 7.4. The best results were obtained when using lower pH value pH = 5. At these conditions the thickness of the fucoidan coating was estimated to about 7 nm.

► This is, to the best of our knowledge, the first report worldwide on low-pressure plasma functionalization of the polymer to improve bonding of fucoidan - a material with antithrombogenic properties.
► A direct correlation between concentration of nitrogen and adsorption of fucoidan was found.
► High resolution angular resolved XPS confirmed that fucoidan adsorbs as an extremely thin coating.