کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1759605 1019285 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Potential mechanism in sonodynamic therapy and focused ultrasound induced apoptosis in sarcoma 180 cells in vitro
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم آکوستیک و فرا صوت
پیش نمایش صفحه اول مقاله
Potential mechanism in sonodynamic therapy and focused ultrasound induced apoptosis in sarcoma 180 cells in vitro
چکیده انگلیسی

Sonodynamic therapy employs a combination of ultrasound and a sonosensitizer to enhance the cytotoxic effect of ultrasound and promote apoptosis. However, the mechanism underlying the synergistic effect of ultrasound and hematoporphyrin is still unclear. In this study, we investigated mechanism of the induction of apoptosis by sonodynamic therapy in Sarcoma 180 cells. The cell suspension was treated by 1.75-MHz focused continuous ultrasound at an acoustic power (ISATA) of 1.4 ± 0.07 W/cm2 for 3 min in the absence or presence of 20 μg/ml hematoporphyrin. The proportion of apoptotic cells was determined by flow cytometry. We then analyzed the reactive oxygen species generation and localization by confocal microscopy. Western blotting and reverse transcriptase-polymerase chain reaction were used to analyze the expression of caspase-8, caspase-9, poly(ADP)-ribose polymerase, and nuclear factor-κB. The findings of our study indicate that ultrasound treatment induced the activation of nuclear factor-κB as an early stress response. When cells were pretreated with hematoporphyrin, the initial response to the therapy was the formation of 1O2 in the mitochondria. Our results primarily demonstrate that the mechanisms of induction of apoptosis by ultrasound and hematoporphyrin-sonodynamic therapies are very different. Our findings can provide a basis for explaining the synergistic effect of ultrasound and hematoporphyrin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasonics - Volume 49, Issue 8, December 2009, Pages 786–793
نویسندگان
, , , , , ,