Layer-by-Layer self-assembly of polyaspartate and Poly(ethyleneimine) on magnetic nanoparticles: Characterization and adsorption of protein

Magnetic nanoparticles (MNPs) were modified by polyaspartate (PAA) and poly(ethyleneimine) (PEI) via Layer-by-Layer self-assembly. Two steps were involved in: the first was to coat MNPs by PAA at pH = 6; the second was to coat the former by PEI. The successful surface modification of MNPs was confirmed using X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA) and fourier transform infrared (FTIR) measurements. The modified MNPs interact strongly with negative fibrinogen and weakly with neutral γ-globin and positive lysozyme.

► Magnetic nanoparticles were modified via Layer-by-Layer self-assembly.
► The nonspecific adsorption of protein on MNPs was resisted. 
►  The modified MNPs interact strongly with negative fibrinogen.
► The modified MNPs interact weakly with neutral γ-globin and positive lysozyme.