A magnetic bead-based bienzymatic electrochemical immunosensor for determination of H9N2 avian influenza virus

• This immunosensor is based on immunomagnetic separation and bienzymatic system.
• HRP modified electrode was fabricated by LBL technique with excellent properties.
• The electrode could be regenerated with a simple washing procedure.
• The detection could be finished in a short time with high sensitivity.
• The proposed immunosensor could be applied to complex samples.

A novel electrochemical immunosensor based on the integration of immunomagnetic separation and bienzymatic amplification for sensitive detection of virus particles was fabricated in this work. The bienzymatic strategy was realized by using the first enzyme as tracer tagged on immunomagnetic beads which could be accumulated on the magneto controlled home-made Au electrode (m-AuE) and the second enzyme immobilized on the m-AuE by layer-by-layer (LBL) assembly technique. The proposed immunosensor not only provides a rapid, simple, cost-effective and on-site platform with high sensitivity, selectivity, and reproducibility for early diagnosis but also presents a new approach for sensitive magneto immunoassay.

Figure optionsDownload as PowerPoint slide