کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
181643 | 459407 | 2008 | 4 صفحه PDF | دانلود رایگان |
Gold nanorods (GNRs) were synthesized by a seed–mediated growth approach followed by TEOS polymerization leading to the formation of silica layer surrounding the gold nanorod core. TEM images showed that the silica-coated gold nanorods (GNRs@SiO2) were dispersed with an average aspect ratio of 3.1 for the GNRs cores and a uniform thickness of the silica shell. The core/shell nanocomposites were further used as efficient supports for the immobilization of hemoglobin (Hb) to fabricate a novel biosensor. The immobilized Hb showed an enhanced electron transfer for its heme Fe(III) to Fe(II) redox couple. This biosensor showed an excellent bioelectrocatalytic activity towards H2O2 with a linear range from 8.0 × 10−7 to 6.1 × 10−5 M, and the detection limit was 6.0 × 10−8 M at 3σ. The apparent Michaelis–Menten constant of the immobilized hemoglobin was calculated to be 0.13 mM.
Journal: Electrochemistry Communications - Volume 10, Issue 3, March 2008, Pages 355–358