کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
182526 459431 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetic study of DNA/DNA hybridization with electrochemical impedance spectroscopy
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Kinetic study of DNA/DNA hybridization with electrochemical impedance spectroscopy
چکیده انگلیسی

The hybridization of immobilized oligonucleotides probe strands with solution phase targets is the underlying principle of microarray-based techniques for the analysis of DNA variation. To study the kinetics of DNA/DNA hybridization, target DNA is often prior labeled with markers. A label-free method of electrochemical impedance spectra (EIS) for study the hybridization in process was reported. The Langmuir model was used to determine the association rate constant (Kon), the dissociation rate constant (Koff) and the affinity rate constant (KA), for perfect matched DNA hybridization. The results show that, EIS is a successful technique possessing high effectivity and sensitivity to study DNA/DNA hybridization kinetics. This work can provide another view on EIS for the studying of DNA/DNA hybridization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochemistry Communications - Volume 9, Issue 2, February 2007, Pages 191–196
نویسندگان
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