کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1876385 | 1042008 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Radiopeptide internalisation and externalisation assays: Cell viability and radioligand integrity
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موضوعات مرتبط
مهندسی و علوم پایه
فیزیک و نجوم
تشعشع
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Radiopeptide internalisation and externalisation assays: Cell viability and radioligand integrity Radiopeptide internalisation and externalisation assays: Cell viability and radioligand integrity](/preview/png/1876385.png)
چکیده انگلیسی
Various aspects of radiopeptide receptor-mediated cell internalisation and externalisation assays were assessed, including the integrity of externalised peptides and the effect of varying the pH and incubation time of the acid wash step (to remove surface receptor-bound ligand) on efficacy and cell viability. The observed intact proportion of externalised peptide was 5–10%, and acid wash buffers with pH 2.8 or below were found to be detrimental to cell viability and integrity, particularly following prolonged incubation times.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Radiation and Isotopes - Volume 69, Issue 1, January 2011, Pages 68–74
Journal: Applied Radiation and Isotopes - Volume 69, Issue 1, January 2011, Pages 68–74
نویسندگان
Syed Ali Raza Naqvi, Jane K. Sosabowski, Saeed Ahamad Nagra, Malik M. Ishfaq, Stephen J. Mather, Torkjel Matzow,