Effect of γ-irradiation on the biology and ultrastructure of haemocytes of greater wax moth, Galleria mellonella (L.) (Lepidoptera: Galleridae)

This study was carried out on fully grown pupae of greater wax moth, Galleria mellonella L., γ-irradiated to 100, 150, 300 and 400 Gy. The four doses given to male parents in the F1 generation decreased the average number of eggs per mated female, the percentage of egg hatching and the percentage of mating in both the male and female lines; the effects increased with the dose. Dose dependence of the reduction in the fecundity and the percentage of egg hatching among the female line pairings (female descendants of irradiated parental male pupae) was more significant than among the male line pairings (male descendants of irradiated parental male pupae). We also examined morphological changes in the irradiated blood cells using transmission electron microscopy (TEM). Vacuolization of the cytoplasm, disorganization and swelling of mitochondria were found.