کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1878256 1042294 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct 99mTc labeling of Herceptin (trastuzumab) by 99mTc(I) tricarbonyl ion
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم تشعشع
پیش نمایش صفحه اول مقاله
Direct 99mTc labeling of Herceptin (trastuzumab) by 99mTc(I) tricarbonyl ion
چکیده انگلیسی

By simply incubating Herceptin (trastuzumab) with [99mTc(CO)3(OH2)3]+ ion in saline, a significant yield of 99mTc-labeled trastuzumab was found to be achievable. The effective labeling may be based on that trastuzumab is inherent with endogenous histidine group to which 99mTc(I) tricarbonyl ion can be strongly bound. For practical 99mTc labeling processing, trastuzumab was purified beforehand from the commercial product, Herceptin (Genentech) via size exclusion chromatography to remove the excipient, α-histidine and a high-labeled yield could be obtained by incubating the purified trastuzumab with [99mTc(CO)3(OH2)3]+. Retention of bioactivity of the 99mTc(I)-labeled trastuzumab was validated using a cell binding test.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Radiation and Isotopes - Volume 66, Issue 3, March 2008, Pages 340–345
نویسندگان
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