کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1886549 1043532 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA binding hydroxyl radical probes
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم تشعشع
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DNA binding hydroxyl radical probes
چکیده انگلیسی

The hydroxyl radical is the primary mediator of DNA damage by the indirect effect of ionizing radiation. It is a powerful oxidizing agent produced by the radiolysis of water and is responsible for a significant fraction of the DNA damage associated with ionizing radiation. There is therefore an interest in the development of sensitive assays for its detection. The hydroxylation of aromatic groups to produce fluorescent products has been used for this purpose. We have examined four different chromophores, which produce fluorescent products when hydroxylated. Of these, the coumarin system suffers from the fewest disadvantages. We have therefore examined its behavior when linked to a cationic peptide ligand designed to bind strongly to DNA.


► Examined four aromatic groups as a means to detect hydroxyl radicals by fluorescence.
► Coumarin system suffers from the fewest disadvantages.
► Characterized its reactivity when linked to a hexa-arginine peptide.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Radiation Physics and Chemistry - Volume 81, Issue 1, January 2012, Pages 46–51
نویسندگان
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