کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1906266 | 1534883 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of cytoplasmic p53 differentially modulates Ca2Â + signaling and cellular viability in young and aged striata
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کلمات کلیدی
aCSFheat shock 70 kDa proteinHSP 70tBHPΔΨmGSHG6PDp53p53 upregulated modulator of apoptosis - modulator modulator آپوپتوزی p53ROS - ROSStriatum - استریاتومelectron transport chain - زنجیره انتقال الکترونAging - سالخوردگیOxygen consumption - مصرف اکسیژنtert-butyl hydroperoxide - هیدروپراکسید تری بوتیلETc - و غیرهMitochondrial membrane potential - پتانسیل غشای میتوکندریPUMA - پوماCalcium - کلسیمGlutathione - گلوتاتیونglutamate - گلوتاماتglucose-6-phosphate dehydrogenase - گلوکز 6-فسفات دهیدروژنازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Inhibition of cytoplasmic p53 differentially modulates Ca2Â + signaling and cellular viability in young and aged striata Inhibition of cytoplasmic p53 differentially modulates Ca2Â + signaling and cellular viability in young and aged striata](/preview/png/1906266.png)
چکیده انگلیسی
The p53 protein, a transcription factor with many gene targets, can also trigger apoptosis in the cytoplasm. The disruption of cell homeostasis, such as Ca2 + signaling and mitochondrial respiration, contributes to the loss of viability and ultimately leads to cell death. However, the link between Ca2 + signaling and p53 signaling remains unclear. During aging, there are alterations in cell physiology that are commonly associated with a reduced adaptive stress response, thus increasing cell vulnerability. In this work, we examined the effects of a cytoplasmic p53 inhibitor (pifithrin μ) in the striatum of young and aged rats by evaluating Ca2 + signaling, mitochondrial respiration, apoptotic protein expression, and tissue viability. Our results showed that pifithrin μ differentially modulated cytoplasmic and mitochondrial Ca2 + in young and aged rats. Cytoplasmic p53 inhibition appeared to reduce the mitochondrial respiration rate in both groups. In addition, p53 phosphorylation and Bax protein levels were elevated upon cytoplasmic p53 inhibition and could contribute to the reduction of tissue viability. Following glutamate challenge, pifithrin μ improved cell viability in aged tissue, reduced reactive oxygen species (ROS) generation, and reduced mitochondrial membrane potential (ÎΨm). Taken together, these results indicate that cytoplasmic p53 may have a special role in cell viability by influencing cellular Ca2 + homeostasis and respiration and may produce differential effects in the striatum of young and aged rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Gerontology - Volume 58, October 2014, Pages 120-127
Journal: Experimental Gerontology - Volume 58, October 2014, Pages 120-127
نویسندگان
Rodrigo Portes Ureshino, Yi-Te Hsu, Lúcia Garcez do Carmo, César Henrique Yokomizo, Iseli Lourenço Nantes, Soraya Soubhi Smaili,