کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1906762 1046314 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Restoration of the behavioral rates and lifespan in clk-1 mutant nematodes in response to exogenous coenzyme Q10
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Restoration of the behavioral rates and lifespan in clk-1 mutant nematodes in response to exogenous coenzyme Q10
چکیده انگلیسی

The clk-1 gene encodes demethoxyubiquinone mono-oxygenase that is necessary for the biosynthesis of coenzyme Q (CoQ), which is an electron transporter in the respiratory chain of mitochondria. Therefore, clk-1 mutant nematodes that have loss-of-function mutations in the clk-1 gene lack endogenous CoQ9 and exhibit slowed behavioral rates and an extended lifespan compared with wild-type animals when they are fed standard bacteria containing endogenous CoQ8. This finding suggests that clk-1 regulates behavioral rates and the lifespan through CoQ in nematodes; however, the effects of exogenous CoQ on the regulation of these biological processes have been incompletely evaluated. In this study, we found that adding 10 μM water-soluble CoQ10 to the culture medium of clk-1 mutant nematodes that were fed a diet of standard bacteria restored the pharyngeal pumping, defecation and the lifespan to levels that were comparable to those of wild-type animals. The results indicate that both behavioral rates and lifespan are regulated by the clk-1 gene through the action of CoQ in nematodes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Gerontology - Volume 47, Issue 3, March 2012, Pages 276–279
نویسندگان
, , , ,