کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1908642 1046677 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Wanted and wanting: Antibody against methionine sulfoxide
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Wanted and wanting: Antibody against methionine sulfoxide
چکیده انگلیسی

Methionine residues in protein can be oxidized by reactive oxygen or nitrogen species to generate methionine sulfoxide. This covalent modification has been implicated in processes ranging from normal cell signaling to neurodegenerative diseases. A general method for detecting methionine sulfoxide in proteins would be of great value in studying these processes, but development of a chemical or immunochemical technique has been elusive. Recently, an antiserum raised against an oxidized corn protein, DZS18, was reported to be specific for methionine sulfoxide in proteins (Arch. Biochem. Biophys. 485:35–40; 2009). However, data included in that report indicate that the antiserum is not specific. Utilizing well-characterized native and methionine-oxidized glutamine synthetase and aprotinin, we confirm that the antiserum does not possess specificity for methionine sulfoxide.


► Methionine oxidation is an important posttranslational modification.
► Lack of assays for methionine sulfoxide in proteins hinders the field.
► An antiserum claiming specificity for methionine sulfoxide was recently reported.
► The report actually shows that the antiserum lacks the claimed specificity.
► We confirm that the antiserum is not specific for methionine sulfoxide.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 53, Issue 6, 15 September 2012, Pages 1222–1225
نویسندگان
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