کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1909864 | 1046743 | 2010 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Aberrant repair of etheno-DNA adducts in leukocytes and colon tissue of colon cancer patients
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کلمات کلیدی
4-hydroxynonenalLPOBERHNE3,N4-ethenocytosine1,N2-ethenoguanine1,N6-ethenoadenine - 1، N6-ethanoadeninebase excision repair - تعمیر پایه پایهOxidative stress - تنش اکسیداتیوFree radicals - رادیکال آزادColorectal cancer - سرطان روده بزرگColon cancer - سرطان کولونLipid peroxidation - پراکسیداسیون لیپیدCRC - کد افزونگی دورهای
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Aberrant repair of etheno-DNA adducts in leukocytes and colon tissue of colon cancer patients Aberrant repair of etheno-DNA adducts in leukocytes and colon tissue of colon cancer patients](/preview/png/1909864.png)
چکیده انگلیسی
To assess the role of lipid peroxidation-induced DNA damage and repair in colon carcinogenesis, the excision rates and levels of 1,N6-etheno-2â²-deoxyadenosine (εdA), 3,N4-etheno-2â²-deoxycytidine (εdC), and 1,N2-etheno-2â²-deoxyguanosine (1,N2-εdG) were analyzed in polymorphic blood leukocytes (PBL) and resected colon tissues of 54 colorectal carcinoma (CRC) patients and PBL of 56 healthy individuals. In PBL the excision rates of 1,N6-ethenoadenine (εAde) and 3,N4-ethenocytosine (εCyt), measured by the nicking of oligodeoxynucleotide duplexes with single lesions, and unexpectedly also the levels of εdA and 1,N2-εdG, measured by LC/MS/MS, were lower in CRC patients than in controls. In contrast the mRNA levels of repair enzymes, alkylpurine- and thymine-DNA glycosylases and abasic site endonuclease (APE1), were higher in PBL of CRC patients than in those of controls, as measured by QPCR. In the target colon tissues εAde and εCyt excision rates were higher, whereas the εdA and εdC levels in DNA, measured by 32P-postlabeling, were lower in tumor than in adjacent colon tissue, although a higher mRNA level was observed only for APE1. This suggests that during the onset of carcinogenesis, etheno adduct repair in the colon seems to be under a complex transcriptional and posttranscriptional control, whereby deregulation may act as a driving force for malignancy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 49, Issue 6, 15 September 2010, Pages 1064-1071
Journal: Free Radical Biology and Medicine - Volume 49, Issue 6, 15 September 2010, Pages 1064-1071
نویسندگان
Tomasz ObtuÅowicz, Alicja Winczura, Elżbieta Speina, Maja Swoboda, Justyna Janik, Beata Janowska, JarosÅaw M. CieÅla, PaweÅ Kowalczyk, Arkadiusz Jawien, Daniel Gackowski, Zbigniew Banaszkiewicz, Ireneusz Krasnodebski, Andrzej Chaber,