کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1910708 1046783 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A pulse-radiolysis approach to fast reductive cleavage of a disulfide bond to uncage enzyme activity
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
A pulse-radiolysis approach to fast reductive cleavage of a disulfide bond to uncage enzyme activity
چکیده انگلیسی

The essential thiol of the enzyme papain has been caged by linking to an aromatic thiol. The resulting caged protein is inactive but enzymatic activity is fully restored upon chemical cleavage of the protective disulfide bond. We have exploited the chemistry of this disulfide bond to uncage papain by pulse radiolysis. We have shown that up to 10% of the enzyme activity can be restored by reductive pulse radiolysis. This approach has been tested on a small-molecule model system, and experiments on this model compound show that pulse radiolysis of the mixed cysteine-aromatic disulfide results in selective reduction of the disulfide bond to generate a thiol in 10–20% yield, consistent with the radiolytically restored activity of the caged papain quantified by the biochemical assay.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 45, Issue 9, 1 November 2008, Pages 1271–1278
نویسندگان
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