کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1915188 | 1535179 | 2009 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Improved detection of incipient vascular changes by a biotechnological platform combining post mortem MRI in situ with neuropathology Improved detection of incipient vascular changes by a biotechnological platform combining post mortem MRI in situ with neuropathology](/preview/png/1915188.png)
The histopathological counterpart of white matter hyperintensities is a matter of debate. Methodological and ethical limitations have prevented this question to be elucidated.We want to introduce a protocol applying state-of-the-art methods in order to solve fundamental questions regarding the neuroimaging–neuropathological uncertainties comprising the most common white matter hyperintensities [WMHs] seen in aging. By this protocol, the correlation between signal features in in situ, post mortem MRI-derived methods, including DTI and MTR and quantitative and qualitative histopathology can be investigated. We are mainly interested in determining the precise neuroanatomical substrate of incipient WMHs. A major issue in this protocol is the exact co-registration of small lesion in a tridimensional coordinate system that compensates tissue deformations after histological processing.The protocol is based on four principles: post mortem MRI in situ performed in a short post mortem interval, minimal brain deformation during processing, thick serial histological sections and computer-assisted 3D reconstruction of the histological sections.This protocol will greatly facilitate a systematic study of the location, pathogenesis, clinical impact, prognosis and prevention of WMHs.
Journal: Journal of the Neurological Sciences - Volume 283, Issues 1–2, 15 August 2009, Pages 2–8