کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1923584 | 1048902 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Midkine-deficient mice delayed degeneration and regeneration after skeletal muscle injury
ترجمه فارسی عنوان
موشهای کمبود میدیکین، پس از آسیب عضلات اسکلتی، دچار انقباض و بازسازی شدند
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Midkine (MK), a heparin-binding growth factor, was previously found to be expressed in the rat myotube-forming stage. We investigated MK gene-deficient (Mdkâ/â) mice in terms of skeletal muscle degeneration and regeneration after injury by bupivacaine injection into the tibialis anterior muscle. Injured muscles showed intense inflammatory cell infiltration. Myotubes, myofibers with centrally located nuclei in their cytoplasm, were significantly smaller in Mdkâ/â mice than in wild type (Mdk+/+) mice 7 days after injury (p = 0.02). The distribution of myotube sizes showed quantitative differences between the two groups at 5 and 7 days, but not at 14 days. Many small myotubes were found in the regenerative area of Mdkâ/â mice compared with that of Mdk+/+mice 5 and 7 days after injury. The expression of Iba1, a macrophage marker, was significantly lower in Mdkâ/â mice 3 days after injury (p = 0.01). The number of desmin-positive cells like myoblasts in Mdkâ/â mice was significantly fewer than that in Mdk+/+ mice 3 days after injury. Our results suggested that deletion of MK results in a delay in regeneration, preceded by decelerated migration of macrophages to the damaged area, and that MK has a role in cell differentiation and maturation after skeletal muscle injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 116, Issue 2, March 2014, Pages 319-326
Journal: Acta Histochemica - Volume 116, Issue 2, March 2014, Pages 319-326
نویسندگان
Masako Ikutomo, Harutoshi Sakakima, Fumiyo Matsuda, Yoshihiro Yoshida,