کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941782 1536905 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrospun bioactive mats enriched with Ca-polyphosphate/retinol nanospheres as potential wound dressing
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Electrospun bioactive mats enriched with Ca-polyphosphate/retinol nanospheres as potential wound dressing
چکیده انگلیسی


• Effect of PLA fiber mats with polyphosphate/retinol nanospheres on gene expression.
• Increased expression of FABP4, leptin and leptin receptor in MC3T3-E1 cells.
• Application as wound dressings, combining antimicrobial and regenerative properties.

BackgroundWhile electrospun materials have been frequently used in tissue engineering no wound dressings exist that significantly improved wound healing effectively.MethodsWe succeeded to fabricate three-dimensional (3D) electrospun poly(D,l-lactide) (PLA) fiber mats into which nanospheres, formed from amorphous calcium polyphosphate (polyP) nanoparticles (NP) and encapsulated retinol (“retinol/aCa-polyP-NS” nanospheres [NS]), had been incorporated.ResultsExperiments with MC3T3-E1 cells revealed that co-incubation of the cells with Ca-polyP together with retinol (or incubation with retinol/aCa-polyP-NS) resulted in a significant synergistic effect on cell growth compared with particle-free polyP complexed with Ca2+ or amorphous Ca-polyP NPs and retinol alone. Incubation of the cells in the presence of the retinol/aCa-polyP NSs also caused a significant increase of the expression levels of the genes encoding for the fatty acid binding protein 4 (FABP4), as well as of the genes encoding for leptin and the leptin receptor. In contrast, the single components, soluble Na-polyP, complexed to Ca2+, or retinol-free aCa-polyP NPs, and retinol, had no significant effect on the expression of these genes.ConclusionsThese results indicate that the PLA fibers, supplemented with aCa-polyP-NP or retinol/aCa-polyP-NS, elicit morphogenetic activity, suggesting that these fiber mats, along with the antibacterial effect of polyP, have a beneficial potential as wound dressings combining antimicrobial and regenerative (wound healing) properties.General significanceThe PLA-based fiber mats, containing retinol and polyP nanoparticles, provide promising bioactive meshes that are urgently needed as dressings for chronic wounds.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 3, September 2015, Pages 150–160
نویسندگان
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