کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1969057 1538870 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of three rapid assays for Mycobacterium tuberculosis complex detection in a comprehensive hospital from West China
ترجمه فارسی عنوان
ارزیابی سه تست سریع برای تشخیص پیچیدگی میکوباکتریوم توبرکلوزیس در یک بیمارستان جامع از غرب چین
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Our study indicates a moderate rapid diagnostic performance for TB in West China.
• Real-time PCR and MTD are reliable for diagnosis and more sensitive than AFB smear.
• Real-time PCR can be preferred for extra-pulmonary tuberculosis and rare samples.
• MTD can be proposed for respiratory specimen to confirm clinical diagnosis.
• Qualified sample and combined detection will improve diagnostic capacity for TB.

ObjectivesTo assess the capacity of rapid and accurate confirmation of the Mycobacterium tuberculosis complex (MTBC) in a Chinese clinical laboratory.Design and methodsThis prospective study investigated three rapid assays, the Amplified Mycobacterium Tuberculosis Direct (MTD) test, real-time PCR, and acid-fast bacilli (AFB) smear, for direct detection of MTBC in a large consecutive series of different clinical specimens. Performance parameters were estimated and compared overall and for separate specimen categories using a combined reference gold standard.ResultsThe overall sensitivities were similar for MTD and real-time PCR (62.26% vs. 58.49%), significantly higher than those of AFB smear (31.13%). Among three assays, MTD had a satisfactory sensitivity in respiratory specimen (73.33%) and a nearly perfect detection for smear-positive samples (96.97%). Real-time PCR showed a high positive rate (58.97%) in regard to nonrespiratory specimen. A combination of molecular assays with conventional methods reached marked additive diagnostic values (sensitivity up to 76.42%), higher than each method individually. All detection systems showed excellent specificities (> 96.00%).ConclusionsThe present study indicated that our lab had a moderate diagnostic performance for tuberculosis. Quality guarantee for specimen pretreatment, as well as combination analysis, will enable these assays to better incorporate into the routine laboratory workflow in China.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Biochemistry - Volume 48, Issues 1–2, January 2015, Pages 79–84
نویسندگان
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