Determination of ganciclovir in human plasma by ultra performance liquid chromatography–UV detection

ObjectivesImplement a sensitive UHPLC method for the assay of ganciclovir in human plasma.Design and methodsWe developed and validated a chromatographic method coupled to ultraviolet detection for quantification of ganciclovir, with a short run time using a small volume of human plasma. Comparison of system performance was made with respect to analysis time, efficiency and sensitivity.ResultsCorrelation coefficients (r) of the calibration curves ranged from 0.999744 to 0.999784. Within-day and between-day imprecision and inaccuracy, specificity and recovery were also evaluated for validation. The method was precise and accurate and the retention time was 0.7 min. The calibration curves were linear between 0.5 and 30 μg/mL. There was a good correlation between HPLC and UHPLC techniques.ConclusionsWe developed a method that is currently applied in a clinical study assessing GCV plasma concentration variability after ganciclovir and valganciclovir administration.

► A method assessing GCV plasma concentration after ganciclovir administration.
► Pharmacokinetic study in renal transplantation patients.
► An approach allowing dose adjustment to achieve a target plasma exposure.
► GCV determination to improve outcome in SOT patients infected by CMV.
► Plasma samples were determined by HPLC and UHPLC and both results were correlated.