Comparison of four current 25-hydroxyvitamin D assays

► The performances of recently developed vitamin D total assays (ADVIA Centaur and Elecsys) were compared to LC-MS/MS and LIASON 25-OH Vitamin D total assay using clinical samples and SRM 972. ► The new, automated total vitamin D assays show acceptable correlation with LC-MS/MS, and could be used in routine laboratories. ► However, standardization of vitamin D assays and consideration of assay-specific decision limits should be addressed.