کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1969560 1059775 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Combination of multiplex PCR and DHPLC-based strategy for CYP2D6 genotyping scheme in Thais
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Combination of multiplex PCR and DHPLC-based strategy for CYP2D6 genotyping scheme in Thais
چکیده انگلیسی

ObjectiveTo develop CYP2D6 genotyping scheme for accurate allele calling and reliable estimation of functional allele dosage in Thais.Design and methodsWe analyzed CYP2D6 copy numbers by pentaplex PCR coupled with semi-quantitative denaturing high performance liquid chromatography (DHPLC)-based technique. Ten common SNPs were genotyped from CYP2D6 gene product using single base extension (SBE) followed by DHPLC analysis. This detection scheme was compared with real-time PCR and conventional PCR-RFLP for cost-effectiveness.ResultsThe distribution of CYP2D6 gene copy numbers in our population ranged from zero (0.69%), one (7.99%), two (60.07%), three (28.13%) and four (3.13%). The most commonly detected SNPs were related to CYP2D6*10 haplotype. CYP2D6*36 in tandem with CYP2D6*10B is the major rearrangement type in Thais (18.75%).ConclusionsMultiplex PCR coupled with DHPLC-based strategy is convenient and reliable method for CYP2D6 genotyping offering sufficient allele coverage for Asians. Both cost and analytical time saving were shown and the method could potentially be modified to accommodate CYP2D6 genotyping in other ethnics.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Biochemistry - Volume 44, Issue 13, September 2011, Pages 1144–1152
نویسندگان
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