Changes in leukocyte glutamate dehydrogenase activity in alcoholics upon break in alcohol consumption

ObjectivesWe wanted to find the influence of alcohol on leukocyte glutamate dehydrogenase (GLDH) activity, its diagnostic value, influence on metabolism and cells toxicity.Design and MethodsIt was assessed three times in 238 alcoholics and once in 244 healthy persons. We developed our own method applying Triton and two freeze–thaw cycles on − 20 °C for defining its activity.ResultsOur reference activity ranges from 0.08 to 1.21 μkat/g. We have confirmed fast increase of leukocyte GLDH activity after break in alcohol consumption. After 24 h, activity increased by 21.8% (median 31.6%), after 7 days by 33% (median 52%), yet after a short interval since last alcohol intake (up to 48 h), it increased by 32% (median 36%).ConclusionsWe estimate that the fast increase is specific for alcohol addiction. Alcohol consumption reduces GLDH activity to some extent. Consecutively it could lead to increased protein production and strengthen of diminished leukocyte protective ability.