کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1969839 1538894 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cell-free miR-24 and miR-30d, potential diagnostic biomarkers in malignant effusions
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Cell-free miR-24 and miR-30d, potential diagnostic biomarkers in malignant effusions
چکیده انگلیسی

ObjectivesThe study was trying to figure out the possible role of cell-free miR-24 and miR-30d as diagnostic biomarkers for malignant effusions.MethodsA total of 110 effusion samples (28 benign and 82 malignant) were collected and cell-free total RNA was extracted. Quantitative RT-PCR was carried out to measure the expression levels of miR-24 and miR-30d, with an exogenous supplemented plant miRNA, ath-miR156a as the reference RNA.ResultsMalignant pleural effusions had higher expression levels of cell-free miR-24 and miR-30d than benign effusions. In the ascites group, only miR-30d was found to be significantly up-regulated in the supernatant of effusions. Combination of cell-free miR-24 and miR-30d could discriminate malignant ascites from benign with improved potency comparing to single miR-30d.ConclusionsCell-free miR-30d and miR-24 are potential diagnostic biomarkers for malignant and benign effusions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Biochemistry - Volume 44, Issues 2–3, February 2011, Pages 216–220
نویسندگان
, , , , , , , , , ,