کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1969839 | 1538894 | 2011 | 5 صفحه PDF | دانلود رایگان |

ObjectivesThe study was trying to figure out the possible role of cell-free miR-24 and miR-30d as diagnostic biomarkers for malignant effusions.MethodsA total of 110 effusion samples (28 benign and 82 malignant) were collected and cell-free total RNA was extracted. Quantitative RT-PCR was carried out to measure the expression levels of miR-24 and miR-30d, with an exogenous supplemented plant miRNA, ath-miR156a as the reference RNA.ResultsMalignant pleural effusions had higher expression levels of cell-free miR-24 and miR-30d than benign effusions. In the ascites group, only miR-30d was found to be significantly up-regulated in the supernatant of effusions. Combination of cell-free miR-24 and miR-30d could discriminate malignant ascites from benign with improved potency comparing to single miR-30d.ConclusionsCell-free miR-30d and miR-24 are potential diagnostic biomarkers for malignant and benign effusions.
Journal: Clinical Biochemistry - Volume 44, Issues 2–3, February 2011, Pages 216–220