Modification of the EMIT immunoassay for the measurement of unbound mycophenolic acid in plasma

ObjectivesEvaluation of the performance of a modified Enzyme Multiplied Immunoassay Technique for therapeutic drug monitoring of plasma free mycophenolic acid (fMPA) concentrations.Design and methodsA fMPA assay was developed on a Viva-E analyzer. A study of prior ultrafiltration conditions and analytical validation of the EMIT assay were performed.ResultsThe method was reliable and reproducible.ConclusionsfMPA levels can be monitored using this EMIT assay with the advantage of being an automated method.