کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1969969 | 1059786 | 2009 | 9 صفحه PDF | دانلود رایگان |
ObjectivesCharacteristic for the genes encoding glutathione S-transferase (GST) M1 and GSTT1 is a null allele, suggested to increase susceptibility to chronic diseases. We report an optimized method for the determination of copy number variation (CNV) in GST genes.Design and methodsReal-time multiplex PCR reactions were optimized for quantification of GSTM1 and GSTT1 CNV using the ΔCt method, a fixed volume of diluted DNA, a total volume of 10 μL, 384-well formats, and single determinations of each sample.ResultsConsistent genotyping was obtained using DNA in a range of 0.41 ng to 100 ng. In a general population sample of 20,687 individuals the genotype frequencies were concordant with other methods used as standards. Throughput was 4600 genotypes per day at a reagent price of 0.5 euros per sample.ConclusionsThis high-throughput, low cost method accurately determines CNV in the GST genes enabling reliable estimates of disease prediction in large epidemiological samples.
Journal: Clinical Biochemistry - Volume 42, Issue 3, February 2009, Pages 201–209